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The primers traditionally used to detectPlasmodium ovale infections are known for not binding allP. ovale parasites within the small-subunit rRNA gene when used alone. We describe a simple, cost- and time-efficient multiplex nested PCR and a nested PCR using a novel set of primers for the simultaneous detection ofP. ovale curtisi andP. ovale wallikeri .

Two Techniques for Simultaneous Identification of Plasmodium ovale curtisi and Plasmodium ovale wallikeri by Use of the Small-Subunit rRNA Gene