Open AccessTwo Techniques for Simultaneous Identification of Plasmodium ovale curtisi and Plasmodium ovale wallikeri by Use of the Small-Subunit rRNA Gene
Author(s) -
HansPeter Fuehrer,
Marie-Therese Stadler,
Katharina Buczolich,
Ingrid Bloeschl,
Harald Noedl
Publication year - 2012
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.02180-12
Subject(s) - plasmodium ovale , biology , gene , multiplex polymerase chain reaction , ribosomal rna , plasmodium (life cycle) , genetics , virology , polymerase chain reaction , microbiology and biotechnology , malaria , plasmodium vivax , plasmodium falciparum , parasite hosting , immunology , world wide web , computer science
The primers traditionally used to detectPlasmodium ovale infections are known for not binding allP. ovale parasites within the small-subunit rRNA gene when used alone. We describe a simple, cost- and time-efficient multiplex nested PCR and a nested PCR using a novel set of primers for the simultaneous detection ofP. ovale curtisi andP. ovale wallikeri .
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom