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The use of nucleic acid amplification tests (NAATs) for the diagnosis ofClostridium (Clostridioides )difficile infection (CDI) leads to overdiagnosis. To improve the clinical specificity of NAATs, there has been a recent interest in using toxin gene cycle thresholds (CT s) to predict the presence and absence of toxins.

Ultrasensitive Detection of Clostridium difficile Toxins Reveals Suboptimal Accuracy of Toxin Gene Cycle Thresholds for Toxin Predictions