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A quantitative high-resolution melt analysis assay was developed to differentiate lymphogranuloma venereum-causing serovars ofChlamydia trachomatis (L1 to L3) from otherC. trachomatis serovars (D to K). The detection limit of this assay is approximately 10 copies per reaction, comparable to the limits of other quantitative-PCR-based methods.

Rapid Determination of Lymphogranuloma Venereum Serovars of Chlamydia trachomatis by Quantitative High-Resolution Melt Analysis (HRMA)