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The recent publication of Zbinden et al. (4) regarding the Vitek 2 colorimetric GN card for the identification of gram- negative nonfermentative bacilli contains data inconsistencies which the authors should have addressed before making rec- ommendations for the use of this product. The authors stated that the 90 isolates tested were "well identified" but that 20/90 (22.2%) were not identified to the species level. Their "gold standard" reference method (partial 16S rRNA gene sequencing) failed to characterize 10 strains beyond the genus level, and the remaining 10 strains could not be resolved between two species. The authors recommended the Vitek 2 GN card for only five nonfermentative taxa—Achromobacter xylosoxidans, Acineto- bacter spp., Burkholderia cepacia complex, Pseudomonas aerugi- nosa, and Stenotrophomonas maltophilia—but the authors lacked sufficient data to contraindicate the use of the card for other species claimed (identified) by this product. While the Vitek 2 GN card claims over 60 nonfermenter species, the authors tested only 35 taxa, of which at least 14 represented unclaimed taxa; all things considered, less than one-third of the claimed species were actually tested. Of the species whose claims were questioned, over 10 were represented by testing only a single strain. These results

New Vitek 2 Colorimetric GN Card for Identification of Gram-Negative Nonfermentative Bacilli