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Xpert-MTB/Rif is one of the most frequently used molecular screening tests for multidrug-resistant tuberculosis worldwide. We report false-negative assay results in the presence of rpoB Leu533Pro, which is associated with low-level phenotypic rifampin resistance. Accurate and timely confirmation of rifampin susceptibility results obtained with Xpert-MTB/Rif is imperative.

journal of clinical microbiology

Diagnostic Implications of Inconsistent Results Obtained with the Xpert MTB/Rif Assay in Detection of Mycobacterium tuberculosis Isolates with an <i>rpoB</i> Mutation Associated with Low-Level Rifampin Resistance

Diagnostic Implications of Inconsistent Results Obtained with the Xpert MTB/Rif Assay in Detection of Mycobacterium tuberculosis Isolates with an rpoB Mutation Associated with Low-Level Rifampin Resistance