Open AccessLong-Term Stability of Human Genomic and Human Papillomavirus DNA Stored in BD SurePath and Hologic PreservCyt Liquid-Based Cytology Media
Author(s) -
Patricia Agreda,
Gerard H. Beitman,
Erin C. Gutierrez,
James Michael Harris,
Kristopher Koch,
William Laviers,
Sharon Leitch,
Courtney E. Maus,
Ray A. McMillian,
William A. Nussbaumer,
Marcus L. R. Palmer,
Michael Porter,
Gregory A. Richart,
Ryan J. Schwab,
Laurence M. Vaughan
Publication year - 2013
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00759-13
Subject(s) - liquid based cytology , cytology , human papillomavirus , dna , dna extraction , genomic dna , virology , microbiology and biotechnology , biology , polymerase chain reaction , medicine , cervical cancer , gene , genetics , cancer
We evaluated the effect of storage at 2 to 8°C on the stability of human genomic and human papillomavirus (HPV) DNA stored in BD SurePath and Hologic PreservCyt liquid-based cytology media. DNA retained the ability to be extracted and PCR amplified for more than 2.5 years in both medium types. Prior inability to detect DNA in archived specimens may have been due to failure of the extraction method to isolate DNA from fixed cells.
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