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A variable-number tandem-repeat genotyping method for Mycobacterium tuberculosis was converted to run in a multiplex PCR format on a 12-well microfluidic laboratory chip. Epidemiologically and genotypically distinct isolate clusters of M. tuberculosis were identified. This rapid genotyping method has potential application in smaller clinical laboratories and public health field investigations.

journal of clinical microbiology

Multiplex Amplified Nominal Tandem-Repeat Analysis (MANTRA), a Rapid Method for Genotyping <i>Mycobacterium tuberculosis</i> by Use of Multiplex PCR and a Microfluidic Laboratory Chip

Multiplex Amplified Nominal Tandem-Repeat Analysis (MANTRA), a Rapid Method for Genotyping Mycobacterium tuberculosis by Use of Multiplex PCR and a Microfluidic Laboratory Chip