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We evaluated whether the Bruker Biotyper matrix-associated laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) system provides accurate species-level identifications of 147 isolates of aerobically growing Gram-positive rods (GPRs). The bacterial isolates includedNocardia (n = 74),Listeria (n = 39),Kocuria (n = 15),Rhodococcus (n = 10),Gordonia (n = 7), andTsukamurella (n = 2) species, which had all been identified by conventional methods, molecular methods, or both. In total, 89.7% ofListeria monocytogenes , 80% ofRhodococcus species, 26.7% ofKocuria species, and 14.9% ofNocardia species (n = 11, allN. nova andN. otitidiscaviarum ) were correctly identified to the species level (score values, ≥2.0). A clustering analysis of spectra generated by the Bruker Biotyper identified six clusters ofNocardia species, i.e., cluster 1 (N. cyriacigeorgica ), cluster 2 (N. brasiliensis ), cluster 3 (N. farcinica ), cluster 4 (N. puris ), cluster 5 (N. asiatica ), and cluster 6 (N. beijingensis ), based on the six peaks generated by ClinProTools with the genetic algorithm, i.e.,m/z 2,774.477 (cluster 1),m/z 5,389.792 (cluster 2),m/z 6,505.720 (cluster 3),m/z 5,428.795 (cluster 4),m/z 6,525.326 (cluster 5), andm/z 16,085.216 (cluster 6). Two clusters ofL. monocytogenes spectra were also found according to the five peaks, i.e.,m/z 5,594.85,m/z 6,184.39, andm/z 11,187.31, for cluster 1 (serotype 1/2a) andm/z 5,601.21 andm/z 11,199.33 for cluster 2 (serotypes 1/2b and 4b). The Bruker Biotyper system was unable to accurately identifyNocardia (except forN. nova andN. otitidiscaviarum ),Tsukamurella , orGordonia species. Continuous expansion of the MALDI-TOF MS databases to include more GPRs is necessary.

journal of clinical microbiology

Bruker Biotyper Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System for Identification of Nocardia, Rhodococcus, Kocuria, Gordonia, Tsukamurella, and Listeria Species