Open AccessRapid Identification of Sporothrix Species by T3B Fingerprinting
Author(s) -
Manoel Marques Evangelista Oliveira,
Paula Sampaio,
Rodrigo AlmeidaPaes,
Célia Pais,
Maria Clara GutierrezGalhardo,
Rosely Maria ZancopéOliveira
Publication year - 2012
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00450-12
Subject(s) - sporothrix , biology , identification (biology) , mycology , species identification , sporotrichosis , microbiology and biotechnology , computational biology , genetics , botany , immunology
This article describes PCR fingerprinting using the universal primer T3B to distinguish among species of the Sporothrix complex, S. brasiliensis, S. globosa, S. mexicana, and S. schenckii. This methodology generated distinct banding patterns, allowing the correct identification of all 35 clinical isolates at the species level, confirmed by partial calmodulin (CAL) gene sequence analyses. This methodology is simple, reliable, rapid, and cheap, making it an ideal routine identification system for clinical mycology laboratories.
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