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Simple, low-cost PCR/PCR-restriction fragment length polymorphism (RFLP) assays targeting cyp51A promoter and codon 98 regions were developed for the detection of triazole-resistant Aspergillus fumigatus strains carrying TR34/L98H mutations. The assays were evaluated using 40 itraconazole-susceptible isolates and 35 itraconazole-resistant isolates. The prevalence of TR34/L98H mutations in clinical/environmental A. fumigatus isolates may now be determined easily from resource-poor settings.

journal of clinical microbiology

Simple, Low-Cost Molecular Assays for TR <sub>34</sub> /L98H Mutations in the <i>cyp51A</i> Gene for Rapid Detection of Triazole-Resistant Aspergillus fumigatus Isolates

Simple, Low-Cost Molecular Assays for TR 34 /L98H Mutations in the cyp51A Gene for Rapid Detection of Triazole-Resistant Aspergillus fumigatus Isolates

Simple, Low-Cost Molecular Assays for TR ₃₄ /L98H Mutations in the cyp51A Gene for Rapid Detection of Triazole-Resistant Aspergillus fumigatus Isolates