Open AccessSimple, Low-Cost Molecular Assays for TR 34 /L98H Mutations in the cyp51A Gene for Rapid Detection of Triazole-Resistant Aspergillus fumigatus Isolates
Author(s) -
Suhail Ahmad,
Ziauddin Khan,
Ferry Hagen,
Jacques F. Meis
Publication year - 2014
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00408-14
Subject(s) - aspergillus fumigatus , itraconazole , biology , gene , restriction fragment length polymorphism , microbiology and biotechnology , polymerase chain reaction , aspergillus , antifungal , genetics
Simple, low-cost PCR/PCR-restriction fragment length polymorphism (RFLP) assays targeting cyp51A promoter and codon 98 regions were developed for the detection of triazole-resistant Aspergillus fumigatus strains carrying TR34/L98H mutations. The assays were evaluated using 40 itraconazole-susceptible isolates and 35 itraconazole-resistant isolates. The prevalence of TR34/L98H mutations in clinical/environmental A. fumigatus isolates may now be determined easily from resource-poor settings.
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