Open AccessAnalyzing Multiclonality of Staphylococcus aureus in Clinical Diagnostics Using spa -Based Denaturing Gradient Gel Electrophoresis
Author(s) -
Andreas Matussek,
Lisa Stark,
Olaf Dienus,
Joakim Aronsson,
Sara Mernelius,
Sture Löfgren,
PerEric Lindgren
Publication year - 2011
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00389-11
Subject(s) - temperature gradient gel electrophoresis , staphylococcus aureus , micrococcaceae , gel electrophoresis , microbiology and biotechnology , biology , bacteria , genetics , 16s ribosomal rna
We present a novel denaturing gradient gel electrophoresis (DGGE) method which characterizes multiclonal communities of Staphylococcus aureus. The spa PCR-based DGGE method simultaneously separates strains that differ in only one base, thereby revealing multiclonal colonization and infections.
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