Open AccessDetection of Carbapenemases by Real-Time PCR and Melt Curve Analysis on the BD Max System
Author(s) -
Marjeta Hofko,
Alexander Mischnik,
Martin Kaase,
Stefan Zimmermann,
Alexander H. Dalpke
Publication year - 2014
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00373-14
Subject(s) - multiplex , melting curve analysis , real time polymerase chain reaction , clinical microbiology , microbiology and biotechnology , biology , multiplex polymerase chain reaction , medicine , polymerase chain reaction , bioinformatics , genetics , gene
We developed a multiplex SYBR green real-time PCR for the BD Max instrument (BD Diagnostics, Sparks, MD) to detect a panel of carbapenemases. The assay was evaluated with 152 consecutive isolates sent to the German National Reference Laboratory, and 65/65 of the carbapenemase-positive and 87/87 of the carbapenemase-negative strains were identified correctly.
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