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Forty-three reference strains involving the 24 most common serovars of Salmonella enterica were examined by using a mass spectrometry-based H antigen typing platform (MS-H). The results indicate that MS-H can be used as a sensitive, rapid, and straightforward approach for the typing of Salmonella flagella at the molecular level without antiserum and phase inversion.

Sequence-Level and Dual-Phase Identification of Salmonella Flagellum Antigens by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)