Open AccessValidation of a Multiplex Real-Time PCR Assay for Detection of Mycobacterium spp., Mycobacterium tuberculosis Complex, and Mycobacterium avium Complex Directly from Clinical Samples by Use of the BD Max Open System
Author(s) -
Talita Trevizani Rocchetti,
Suzane Silbert,
Alicia Gostnell,
Carly Kubasek,
Raymond Widen
Publication year - 2016
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00241-16
Subject(s) - mycobacterium tuberculosis complex , mycobacterium tuberculosis , mycobacterium , multiplex , multiplex polymerase chain reaction , microbiology and biotechnology , biology , mycobacterium avium complex , polymerase chain reaction , tuberculosis , nontuberculous mycobacteria , virology , bacteria , medicine , gene , pathology , genetics
A multiplex real-time PCR was validated on the BD Max open system to detect differentMycobacterium tuberculosis complex,Mycobacterium avium complex, andMycobacterium spp. directly from clinical samples. The PCR results were compared to those with traditional cultures. The multiplex PCR assay was found to be a specific and sensitive method for the rapid detection of mycobacteria directly from clinical specimens.
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