Open AccessIdentification of Streptococcus agalactiae Isolates from Various Phylogenetic Lineages by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry
Author(s) -
Marie-Frédérique Lartigue,
Geneviève Héry-Arnaud,
Eve Haguenoer,
Anne-Sophie Domelier,
Pierre-Olivier Schmit,
Nathalie van der Mee-Marquet,
Philippe Lanotte,
Laurent Méreghetti,
Markus Kostrzewa,
Roland Quentin
Publication year - 2009
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00175-09
Subject(s) - streptococcus agalactiae , biology , mass spectrometry , serotype , multilocus sequence typing , phylogenetic tree , typing , microbiology and biotechnology , genetics , streptococcus , genotype , bacteria , chemistry , chromatography , gene
Variations in proteins related to bacterial diversity may affect species identification performed using matrix-assisted laser desorption ionization (MALDI)-time of flight mass spectrometry. Using this method, we identified 110 Streptococcus agalactiae isolates characterized by serotyping and multilocus sequence typing. Serotype III and sequence type 23 strains expressed the widest variation in molecular weight of putative "species-identifying" biomarker ions. Recognition of the diversity of MALDI patterns observed in strains that represent all major intraspecies lineages assists in the constitution of an optimal reference database.
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