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We evaluated a real-time quantitative PCR combined with a multiplex PCR assay for the quantification of Streptococcus pneumoniae and the simultaneous detection of drug-resistant genes by gel-based PCR, using purulent sputum samples. This assay correctly quantified S. pneumoniae and identified their penicillin and erythromycin susceptibilities directly from samples within 3 h.

journal of clinical microbiology

Rapid Identification of Penicillin and Macrolide Resistance Genes and Simultaneous Quantification of <i>Streptococcus pneumoniae</i> in Purulent Sputum Samples by Use of a Novel Real-Time Multiplex PCR Assay

Rapid Identification of Penicillin and Macrolide Resistance Genes and Simultaneous Quantification of Streptococcus pneumoniae in Purulent Sputum Samples by Use of a Novel Real-Time Multiplex PCR Assay