Open AccessEvaluation of PNA-FISH Yeast Traffic Light for Rapid Identification of Yeast Directly from Positive Blood Cultures and Assessment of Clinical Impact
Author(s) -
Neil Stone,
Rebecca Gorton,
Kevin R. Barker,
P. Ramnarain,
C Kibbler
Publication year - 2013
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.00028-13
Subject(s) - yeast , microbiology and biotechnology , corpus albicans , candida tropicalis , biology , candida parapsilosis , candida krusei , candida albicans , fish <actinopterygii> , fungemia , candida glabrata , antifungal , biochemistry , fishery
The PNA-FISH Yeast Traffic Light assay was performed on 54 clinical isolates of yeasts inoculated into blood culture bottles. The assay showed high sensitivity (Candida albicans/C. parapsilosis, 100%; C. glabrata/C. krusei, 92.3%; C. tropicalis, 100%) and specificity (C. albicans/C. parapsilosis, 100%; C. glabrata/C. krusei, 94.8%; C. tropicalis, 100%). Case note review estimated a change in therapy in 29% of cases had the PNA-FISH result been available to the clinician.
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