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attention appears to have been paid to the cation levels of the incubation medium. Furthermore, no uniform opinion exists about the importance of the erythrocyte concentration. B. Wiegershausen (Arch. Intern. Pharmacodyn. 136:174, 1962) found some influence of cell concentration in the case of the rabbit, cat, and rat, but none in the case of sheep and cattle. A. A. Marucci (J. Bacteriol. 86:1182, 1963) found no influence of the number of rabbit cells originally present in the reaction mixture. From his curves, it is seen that he used relatively high a-toxin concentrations.

Influence of erythrocyte concentration and cation levels on the hemolytic action of staphylococcal alpha-hemolysin