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A study of optimal thymine and deoxythymidine (dThd) growth requirements of the thymineless mutants ofEscherichia coli 15,E. coli 70–462 (strain 70), and a variant,E. coli 70V3–462 (strain 70V3), showed that for maximal turbidity (growth) strain 70 required 10-fold greater concentrations of thymine or dThd than did strain 70V3. On suboptimal concentrations of thymine or dThd, growth of strain 70 was greater on dThd than on thymine. In contrast, maximal growth of strain 70V3 was the same on equimolar concentrations of thymine and dThd. Growth rate of strain 70V3 was the same on equimolar concentrations of thymine and dThd up to 4 μm ; at concentrations of 5 μm and greater, the “4-hr” growth was lower on dThd than on corresponding concentrations of thymine. Cultures of both thymineless mutants synthesized equal maximal amounts of DNA. Whereas strain 70V3 incorporated a maximum of 90% of the thymine or dThd in the media, strain 70 incorporated a maximum of only 10%. This poor utilization by strain 70 was neither a result of thymine or dThd conversion to a low-molecular-weight thymine derivative nor the production of a nonthymine inhibitory substance. Since strains 70 and 70V3 exhibited no thymidylate synthetase activity, the first mutation (strain 15 to strain 70) resulted in the loss of this activity. The second mutation (strain 70 to strain 70V3) probably brought about the loss of an enzyme(s) that catabolizes deoxyribose phosphate, permitting a greater net synthesis of dThd from thymine.

journal of bacteriology

Metabolism of Thymineless Mutants of <i>Escherichia coli</i> I. Absence of Thymidylate Synthetase Activity and Growth Characteristics of Two Sequential Thymineless Mutants

Metabolism of Thymineless Mutants of Escherichia coli I. Absence of Thymidylate Synthetase Activity and Growth Characteristics of Two Sequential Thymineless Mutants