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Strains of Escherichia coli with metabolic blocks affecting early (common) reactions in the pathway of biosynthesis of aromatic compounds may require for optimal growth in a glucosemineral salts medium the addition of phenylalanine, tyrosine, tryptophan, p-aminobenzoate, p-hydroxybenzoate, and 3,4-dihydroxybenzaldehyde (B. D. Davis, J. Bacteriol. 64:729, 1952; B. D. Davis, Congr. Intern. Biochim., 2nd, Paris, Symp. Metabolisme Microbien, p. 32, 1952). Some strains with blocks in the common pathway (multiple aromatic auxotrophs) grow only poorly, or not at all, in the medium supplemented as above unless a small amount of yeast extract is added (e.g., J. Pittard and B. J. Wallace, J. Bacteriol. 91:1494, 1966). It has now been found that 2, 3-dihydroxybenzoate will replace the requirement for yeast extract. Figure 1 shows a dose-response curve with 2, 3-dihydroxybenzoate and E. coli 83-1 as the test organism in shaken cultures. This organism was derived from E. coli W, but the E. coli K-12 strains described by Pittard and Wallace

2,3-Dihydroxybenzoic acid, a new growth factor for multiple aromatic auxotrophs