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Wolstenholme, David R. (Max-Planck-Institut für Biologie, Tübingen, Germany),Cornelius A. Vermeulen, and Gerhardus Venema . Evidence for the involvement of membranous bodies in the processes leading to genetic transformation inBacillus subtilis . J. Bacteriol.92: 1111–1121. 1966.—Data obtained from electron microscopic autoradiographs of profiles of cells of aBacillus subtilis population exposed to H3 -thymidine-labeled donor deoxyribonucleic acid (DNA) during the phase of maximal competence indicated that molecules originating from absorbed DNA are closely associated with membranous bodies, particularly with those situated in the cytoplasm, but that most if not all of the radioactive molecules are outside the bodies. It is suggested that membranous bodies produce enzymes essential to the eventual incorporation of transforming DNA into the bacterial genome, or to the breakdown and utilization or expulsion of absorbed DNA not incorporated as transformant (or to both processes). During the phase of maximal competence, the total number of membranous bodies seen in profiles increased continuously to as much as 2.3 times the numbers found during earlier stages of culture. This increase was not accounted for by a decrease in bacterial cell volume, but resulted from an actual increase in total volume of membranous bodies. The number of membranous bodies visibly connecting plasma membrane and nuclear region increased during maximal competence to as much as 30 times the numbers found in earlier stages. As both increases were found in the absence of donor DNA and only began after maximal competence was attained, it seemed most probable that they were an expression of a physiological state influenced by the continuing deficiency of nutrients in the growth medium during this phase of culture.

Evidence for the Involvement of Membranous Bodies in the Processes Leading to Genetic Transformation in Bacillus subtilis