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Hurwitz, Charles (Veterans Administration Hospital, Albany, N.Y.), and Carmen L. Rosano. Evidence for a streptomycin permease. J. Bacteriol. 90:1233-1237. 1965.-The hypothesis that an induced streptomycin permease is required for entry of the antibiotic into cells is further supported by the finding that proflavine and borate, which inhibit transcription, also block the lethal action of streptomycin. Furthermore, if ribonucleic acid (RNA) synthesis is permitted to proceed in the presence of streptomycin and chloramphenicol, and chloramphenicol is then replaced with either proflavine or borate, these inhibitors of transcription no longer block the lethal action of streptomycin. This finding is interpreted to mean that, if induced messenger RNA for streptomycin-permease is formed before transcription is blocked, inhibitors of transcription no longer block the lethal action of streptomycin by preventing formation of the permease.

Evidence for a Streptomycin Permease