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The researches of Tanner and Dack (1922) and of Meyer (1922) and his collaborators have shown beyond question that B. botulinus is widely distributed in the soil and that its presence therein can be proven by testing the toxicity of mixed cultures made in a peptic digest medium. Our own first attempts to demonstrate toxin forming anaerobes in the soil, using glucose peptone broth in the constricted tube with marble seal, were total failures although pure culture controls of B. botulinus and B. tetini were always highly toxic. We finally showed that the failures were due to the lack of sufficient buffer in our glucose broth, so that fermentative bacteria present in the soils destroyed the toxin as fast as formed and perhaps to some extent inhibited growth of the anaerobes in question. Owing to the well known high resistance of botulinus toxin to acid alone it became evident that some other factor, incidental to growth, and other than acid, but dependent upon it, was responsible. At any rate, the inclusion of ground meat enabled us to secure filtrates of high toxicity from soil cultures to which we had added pure cultures of B. botulinus (Hall and Peterson, 1923) and B. tetani.2 We believe that the value of Meyer's peptic digest medium resides

THE DETECTION OF BACILLUS BOTULINUS AND BACILLUS TETANI IN SOIL SAMPLES BY THE CONSTRICTED TUBE METHOD