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distinguished from the color of fluorescein isothiocyanate. Figure 1 shows the mycelium and the cell walls stained by the method described. The staining reaction of these preparations could be observed as far as a 1:16 dilution of the antiserum. These results suggest that a-amylase may be located on the cell surfaces where the antibody molecules were accessible, although there is no information concerning the site where the exocellular enzyme is formed.

GROUPING OF BETA-HEMOLYTIC STREPTOCOCCI ON CELLULOSE ACETATE MEMBRANES