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Knowledge of viral multiplication in the enteric tract of man or animals, particularly in the case of poliovirus, is derived from studies of the excretion of orally fed virus or the distribution of virus in segments of intestinal and associated visceral tissues removed at autopsy. The development of attenuated poliovirus vaccines and the related problems of altered virulence through serial intestinal passage has increased the need for a method in vitro for investigating the ecology and pathogenesis of viral infections in intact alimentary regions. If resected segments of monkey ileum could survive long enough in a nutrient medium to support virus multiplication, such preparations might facilitate investigation of such problems as infective dosage for virulent and avirulent strains, localization of the site of virus multiplication, recombination among the Enteroviruses in an alimentary environment, or the role of metabolic state of the tissue on intestinal virus carriage. In the following, we shall describe a method for the prolonged maintenance of monkey and human ileum segments in vitro and the multiplication of poliovirus within the wall of such tissues when this agent is introduced into the preparation.

MAINTENANCE OF INTESTINAL TISSUE IN VITRO AND THE MULTIPLICATION OF POLIOVIRUS IN EXCISED SEGMENTS OF MONKEY INTESTINAL TRACT