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The influence of temperature on both the growth of microorganisms and the activity of their enzymes is well known, but its effect on the synthesis of different enzymes has not been extensively investigated. Gale (1940), in studies on decarboxylation of amino acids, observed that washed suspensions of Escherichia coli grown at 27 C were more active than those from cultures grown at 37 C. The loss by a pseudomonad culture of its ability to liquefy gelatin when the temperature was raised has been reported (Greene and Jezeski, 1954). Nashif and Nelson (1953) found little lipase activity in cultures of Pseudomonas fragi which were grown at 30 C even though high counts were obtained. Alford and Elliott (1960) have shown that elevated temperatures inhibit the production of lipase rather than its activity since the enzyme has its optimal activity around 45 C. Stuart et al. (1942) found that many coliforms grew in the Eijkman test at 45.5 C without acid or gas production, and Tittsler (1931) found that temperatures above 34 C inhibited H2S production by Salmonella pullorum. In spite of these indications of a temperature effect on the synthesis of enzyme systems, the usual recommendation for incubation of routine tests for the identification of bacteria is that they be held at or near their optimal temperature (SAB, 1957). This temperature is usually defined as the one at which most rapid growth occurs (Oginsky and Umbreit, 1959). The recent development of quicker methods for the identification of bacterial cultures (Smith and Goodner, 1958), which depend upon rapid growth for their maximal usefulness, emphasizes the need for more detailed information on the effect of temperature on biochemical activity. The work reported here indicates that with several biochemical tests a false negative reaction may occur at optimal incubation temperatures. MATERIALS AND METHODS

EFFECT OF INCUBATION TEMPERATURE ON BIOCHEMICAL TESTS IN THE GENERA PSEUDOMONAS AND ACHROMOBACTER