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Studies on the growth of Mycobacterium tuberculosis in a semisynthetic medium have indicated that this microorganism has no essential requirement for any of the amino acids although growth of most strains is stimulated by glutamic acid (Middlebrook et al., 1954). However, when M. tuberculosis is inhibited by p-aminosalicylic acid (PAS) an exogenous source of methionine is required for restoration of growth since synthesis of this amino acid appears to be blocked by the inhibitor (Hedgecock, 1956). In the present investigation a study has been made of the ability of structurally related amino acids to antagonize methionine under conditions of PASinhibition wherein M. tuberculosis was dependent upon an exogenous source of methionine for growth.

ANTAGONISM OF METHIONINE IN AMINOSALICYLATE-INHIBITION OF MYCOBACTERIUM TUBERCULOSIS