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In 1942, Bernheimer et al., described a partially defined medium which supported growth of the ,3-hemolytic streptococcus. Starting with this medium, Adams and Roe (1945) devised a chemically defined medium which was adequate for the culture of pneumococcus. Improvement in the medium of Adams and Roe (Hoeprich, 1955) resulted from: omission of copper and calcium; addition of L-tyrosine; increase in concentration of iron, potassium, phosphate, glucose, glutamine, asparagine, uracil, adenine, choline, pantothenate, pyridoxine, riboflavin, thiamin, nicotinic acid, and biotin; decrease in concentration of zinc and manganese. Except for the acid hydrolyzate of casein, all the constituents of this improved medium are chemically defined. This medium is completely dialyzable and supports luxuriant growth of the pneumococcus. These attributes make it the medium of choice for metabolic studies of the pneumococcus, such as C14 labeling of pneumococci (Hoeprich, 1955). For the preparation of pneumococcal capsular polysaccharides this medium is unsurpassed. In this report, data are presented evaluating this medium with regard to size of inoculum and repeated subculture. Also, the effect was measured of increasing glucose concentration in the medium after the logarithmic phase of growth had been attained.

EVALUATION OF AN IMPROVED CHEMICALLY DEFINED MEDIUM FOR THE CULTURE OF DIPLOCOCCUS PNEUMONIAE