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The ability of Micrococcus pyogenes var. aureus to produce certain toxins and enzymes has been correlated with the causation of disease in animals and human beings. Various laboratory procedures have been devised to determine the activity of these substances as an index of potential pathogenicity. It is known that nonpathogenic strains may exhibit such activity but to a lesser degree. Therefore, a quantitative estimation of the activity of a toxin or enzyme is desirable in determining presumptive pathogenicity. The most commonly determined substances include coagulase, a-hemolysin, and phosphatase. Of these, phosphatase has received the least attention in regard to quantitative study. The purpose of this paper is to present a quantitative study of the phosphatase activity of a strain of 11. pyogenes var. aureus and to propose a simple rapid technique for measuring the activity of the

A QUANTITATIVE STUDY OF THE PHOSPHATASE ACTIVITY OF MICROCOCCUS PYOGENES