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Experiments indicate that bacteria Escherichia coli, strains B and B/r, inactivated by ultraviolet irradiation, can be reactivated by exposure to light (Kelner, 1949). In addition, E. coli, strain B, can be reactivated by heat (Roberts and Aldous, 1949; Anderson, 1951). Catalase, when added after ultraviolet irradiation, can reactivate E. coli, stIain K-12 (Latarjet and Caldas, 1952). Furthermore, it has been demonstrated that heat reactivation of E. coli, strain B/r, after photosensitized inactivation, can be increased by the addition of certain metabolites to the incubation medium (Heinmets et al., 1952). It is obvious that biological injury resulting from ultraviolet irradiation should be interpreted through the photochemical alterations produced within the cellular structures and metabolites. Photochemical reactions produced by ultraviolet irradiations, in general, can be rieversible or irreversible. FoI example, it is well known that many photooxidative processes can be reversed by subsequent chemical reduction. Consequently, it may be assumed that some of the photochemical alterations produced within the bacterial cell by ultraviolet irradiation could be reversed. Thus, a partial restoration of the biological properties of the cell, corresponding to the reversible fraction of the photochemical reactions, could take place. Such recovery may depend on the magnitude of the total alterations produced by irradiation in the cell, and on the ratio between the reversible and the irreveisible fractions of such alterations. If suitable means are applied to reverse the reversible fraction of cellular injury, provided that the residual injury is in the range of tolerance, the cell may survive. Such a recovery phenomenon would not be restricted necessarily to irradiation injury, but possibly may apply in principle to all chemical and physical injuries

REACTIVATION OF ULTRAVIOLET INACTIVATED ESCHERICHIA COLI BY PYRUVATE