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The apparatus used to supply the cathode radiation was a high voltage electrostatic generator of the Van de Graaff type (Trump and Van de Graaff, 1948; Proctor and Goldblith, 1951), which was operated at a constant potential of about 2.8 megavolts and with a beam current of 2.5 microamperes. A dose of 10,000 rep was delivered in about 1 sec. The salts used were of the reagent or cp grade. The organism used in this study was M. pyogenes var. aureus, strain F.D.A. 209-P. It was grown at 37 C on slants of nutrient agar (Difco) for the required length of time, usually about 24 hours. A uniform suspension of the cocci was prepared in sterile distilled water. Five ml portions of the samples were transferred aseptically to special glass irradiation dishes (each covered with a thin strip of aluminum foil) and irradiated immediately. Each sample received a dose of 10,000 rep delivered in about 1 second. Immediately following irradiation, the control samples and the irradiated samples were diluted to 1:100 with sterile distilled water. Additional dilutions were made as required, and plating in duplicate with nutrient agar (Difco) was carried out as quickly as possible. Plates were incubated at 37 C for 1 day, after which colonies were counted with the aid of a Quebec colony counter.

EFFECTS OF SOME SALTS ON THE GERMICIDAL ACTION OF HIGH VOLTAGE CATHODE RAYS TOWARDS MICROCOCCUS PYOGENES VAR. AUREUS