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Our study of the physiology of the several bacteria considered in Part I; viz., (1) Phytomonas tumefaciens, (2) Phytomonas rhizogenes and (3) Bacillus radiobacter has been continued. Since any important variations in the physiology of these organisms might have an important bearing upon the reactions they induce in plant tissue, an attempt was made to discover any basic differences in their activity. The present investigation has been concerned primarily with three lines of study. An attempt has been made (1) to extend available information on the physiological and pathogenic properties of the organisms and to observe any correlation between physiological behavior and pathogenicity; (2) to study variations in the several organisms as they might be induced by continuous cultivation in certain media under favorable and unfavorable conditions and (3) to determine the effect of successive passages through plants upon the physiological characteristics and virulence of the crown-gall organism.

Studies on Certain Physiological Characters of Phytomonas tumefaciens, Phytomonas rhizogenes, and Bacillus radiobacter