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Oxidation-reduction potentials developed in cultures of Esch. coli have been reported by Cannan, Cohen, and Clark (1928), Hewitt (1931) and Boyd and Reed (1931). Boyd and Reed presented evidence that both gaseous (hydrogen) and nongaseous electromotively active systems are responsible for the potentials observed at different periods of growth in anaerobic cultures of Esch. coli. The studies to be reported here were confined to the potentials developed in stationary and continuous flow cultures of Esch. coli in peptone, a somewhat less complex medium than those employed by other investigators. It has been reported (Clifton, 1933a) that broth cultures of Staphylococcus aureus in or near the maximum stationary growth phase reduce potassium ferricyanide at a rate in the neighborhood of 6.6 X 10-14 millimols per cell per minute. These studies have been extended to the rates of reduction of ferricyanide at different stages of growth in stationary and continuous flow cultures of Esch. coli. Studies on the influence of the concentration of peptone, ferricyanide and organisms on the rate of reduction of ferricyanide in suspensions of "resting" Esch. coli, as outlined in a preliminary paper by one of us (Clifton, 1933b), are also presented.

Oxidation-reduction Potentials and Ferricyanide Reducing Activities in Peptone Cultures and Suspensions of Escherichia coli