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The production of ammonia from various substrata serves, in many cases, as an important character in the identification of species of bacteria. The detection of ammonia as used by Thomas (1912), was first recommended by Ayers, Rupp, and Mudge (1921) and later by Hucker and Wall (1922) as having certain advantages over Nessler's reagent. The reagents necessary in the Thomas test may be used in the presence of glucose, protein, and other organic compounds which generally make up the nutrient media in common use in the laboratory. In the Manual of Methods for Pure Culture Study of Bacteria by the Society of American Bacteriologists the reagents for the Thomas test are listed as follows: I. A 5 per cent solution of phenol. II. A solution of sodium hypochlorite containing 1 per cent available chlorine. To obtain this amount of available chlorine the solution should be so adjusted that 1 cc. of it should neutralize 2.82 cc. of a N/10 solution of sodium thiosulphate (i.e., 24.8 grams to the liter), titrating with starch as an indicator in the presence of acetic acid and potassium iodide. In carrying out this technic in the laboratory, difficulty is often encountered in maintaining a sodium hypochlorite of the proper concentration of available chlorine. Hypochlorite preparations are rarely stable and gradually undergo changes with the loss of available chlorine. For the Thomas test, stock solutions of chlorinated lime are sometimes used similar to the solutions available as disinfectants.' It is necessary to standardize these hypochlorite solutions before use in order to adjust the final solution to the proper concentration. Frequently it is found that

The Detection of Ammonia Production by Bacteria in Agar Slants