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Undirected mismatch repair initiated by the incorporation of the base analog 2-aminopurine kills DNA-methylation-deficient Escherichia coli dam cells by DNA double-strand breakage. Subsequently, the chromosomal DNA is totally degraded, resulting in DNA-free cells.

Generation of DNA-Free Escherichia coli Cells by 2-Aminopurine Requires Mismatch Repair and Nonmethylated DNA