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The type III secretion signal of Yersinia enterocolitica YopN was mapped using a gene fusion approach. yopN codons 1 to 12 were identified as critical for signal function. Several synonymous mutations that abolish secretion of hybrid proteins without altering the codon specificity of yopN mRNA were identified.

The Secretion Signal of YopN, a Regulatory Protein of the Yersinia enterocolitica Type III Secretion Pathway