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TheEscherichia coli gcv operon is transcriptionally regulated by the GcvA, GcvR, Lrp, and PurR proteins. In this study, the cyclic AMP (cAMP) receptor protein (CRP) is shown to be involved in positive regulation of thegcv operon. Acrp deletion reduced expression of agcvT-lacZ fusion almost fourfold in glucose minimal (GM) medium. The phenotype was complemented by both the wild-typecrp gene and fourcrp alleles that encode proteins with amino acid substitutions in known activating regions of CRP. AcyaA deletion also resulted in a fourfold decrease ingcvT-lacZ expression, and wild-type expression was restored by the addition of cAMP to the growth medium. AcyaA crp double deletion resulted in levels ofgcvT-lacZ expression identical to those observed with either single mutation, showing that CRP and cAMP regulate through the same mechanism. Growth in GM medium plus cAMP or glycerol minimal medium did not result in a significant increase ingcvT-lacZ expression. Thus, the level of cAMP present in GM medium appears to be sufficient for regulation by CRP. DNase I footprint analysis showed that CRP binds and protects two sites centered at bp −313 (site 1) and bp −140 (site 2) relative to the transcription initiation site, but a mutational analysis demonstrated that only site 1 is required for CRP-mediated regulation ofgcvT-lacZ expression. Expression of thegcvT-lacZ fusion in acrp gcvA double mutant suggested that CRP’s role is dependent on the GcvA protein.

The Cyclic AMP Receptor Protein Is Dependent on GcvA for Regulation of the gcv Operon