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Bacillus subtilis cwlD anddacB mutants produce spore peptidoglycan (PG) with increased cross-linking but with little change in spore core hydration compared to the wild type. AcwlD dacB double mutant produced spores with a two- to fourfold greater increase in PG cross-linking and novel muropeptides containing glycine residues but no significant changes in spore resistance or core hydration.

Spore Peptidoglycan Structure in a cwlD dacB Double Mutant of Bacillus subtilis