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No periplasmic binding protein has been demonstrated for the ATP-binding cassette (ABC)-type cobalamin transporter BtuCD. New mutations (btuF) are described that affect inner-membrane transport. The BtuF protein has a signal sequence and resembles the periplasmic binding proteins of several other ABC transporters.

journal of bacteriology

A New Class of Cobalamin Transport Mutants ( <i>btuF</i> ) Provides Genetic Evidence for a Periplasmic Binding Protein in <i>Salmonella typhimurium</i>

A New Class of Cobalamin Transport Mutants ( btuF ) Provides Genetic Evidence for a Periplasmic Binding Protein in Salmonella typhimurium