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A DNA fragment containing thePseudomonas aeruginosa fabD (encoding malonyl-coenzyme A [CoA]:acyl carrier protein [ACP] transacylase),fabG (encoding β-ketoacyl-ACP reductase),acpP (encoding ACP), andfabF (encoding β-ketoacyl-ACP synthase II) genes was cloned and sequenced. Thisfab gene cluster is delimited by theplsX (encoding a poorly understood enzyme of phospholipid metabolism) andpabC (encoding 4-amino-4-deoxychorismate lyase) genes; thefabF andpabC genes seem to be translationally coupled. ThefabH gene (encoding β-ketoacyl-ACP synthase III), which in most gram-negative bacteria is located betweenplsX andfabD , is absent from this gene cluster. A chromosomal temperature-sensitivefabD mutant was obtained by site-directed mutagenesis that resulted in a W258Q change. A chromosomalfabF insertion mutant was generated, and the resulting mutant strain contained substantially reduced levels ofcis -vaccenic acid. Multiple attempts aimed at disruption of the chromosomalfabG gene were unsuccessful. We purified FabD as a hexahistidine fusion protein (H6 -FabD) and ACP in its native form via an ACP-intein-chitin binding domain fusion protein, using a novel expression and purification scheme that should be applicable to ACP from other bacteria. Matrix-assisted laser desorption–ionization spectroscopy, native polyacrylamide electrophoresis, and amino-terminal sequencing revealed that (i) most of the purified ACP was properly modified with its 4′-phosphopantetheine functional group, (ii) it was not acylated, and (iii) the amino-terminal methionine was removed. In an in vitro system, purified ACP functioned as acyl acceptor and H6 -FabD exhibited malonyl-CoA:ACP transacylase activity.

journal of bacteriology

Characterization of a <i>Pseudomonas aeruginosa</i> Fatty Acid Biosynthetic Gene Cluster: Purification of Acyl Carrier Protein (ACP) and Malonyl-Coenzyme A:ACP Transacylase (FabD)

Characterization of a Pseudomonas aeruginosa Fatty Acid Biosynthetic Gene Cluster: Purification of Acyl Carrier Protein (ACP) and Malonyl-Coenzyme A:ACP Transacylase (FabD)