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A set of open reading frames (ORFs) potentially encoding signal transduction proteins are clustered around icfG , a gene implicated in the regulation of carbon metabolism, in the genome of Synechocystis sp. strain PCC 6803. slr1860 is the ORF for icfG , whose predicted product resembles the protein phosphatases SpoIIE, RsbU, and RsbX from Bacillus subtilis . Bracketing slr1860/icfG are (i) ORF slr1861 , whose predicted product resembles the SpoIIAB, RsbT, and RsbW protein kinases from B. subtilis , and (ii) ORFs slr1856 and slr1859 , whose predicted products resemble the respective phosphoprotein substrates for the B. subtilis protein kinases: SpoIIAA, RsbS, and RsbV. In order to determine whether the protein products encoded by these ORFs possessed the functional capabilities suggested by sequence comparisons, each was expressed in Escherichia coli as a histidine-tagged fusion protein and analyzed for its ability to participate in protein phosphorylation-dephosphorylation processes in vitro. It was observed that ORF slr1861 encoded an ATP-dependent protein kinase capable of phosphorylating Slr1856 and, albeit with noticeably lower efficiency, Slr1859. Site-directed mutagenesis suggests that Slr1861 phosphorylated these proteins on Ser-54 and Ser-57, respectively. Slr1860 exhibited divalent metal ion-dependent protein-serine phosphatase activity. It catalyzed the dephosphorylation of Slr1856, but not Slr1859, in vitro.

The icfG Gene Cluster ofSynechocystis sp. Strain PCC 6803 Encodes an Rsb/Spo-Like Protein Kinase, Protein Phosphatase, and Two Phosphoproteins