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The most abundant carbon source transported into legume root nodules is photosynthetically produced sucrose, yet the importance of its metabolism by rhizobia in planta is not yet known. To identify genes involved in sucrose uptake and hydrolysis, we screened aSinorhizobium meliloti genomic library and discovered a segment ofS. meliloti DNA which allowsRalstonia eutropha to grow on the α-glucosides sucrose, maltose, and trehalose. Tn5 mutagenesis localized the required genes to a 6.8-kb region containing five open reading frames which were namedagl , for α-glucoside utilization. Four of these (aglE ,aglF ,aglG , andaglK ) appear to encode a periplasmic-binding-protein-dependent sugar transport system, and one (aglA ) appears to encode an α-glucosidase with homology to family 13 of glycosyl hydrolases. Cosmid-borneagl genes permit uptake of radiolabeled sucrose intoR. eutropha cells. Analysis of the properties ofagl mutants suggests thatS. meliloti possesses at least one additional α-glucosidase as well as a lower-affinity transport system for α-glucosides. It is possible that the Fix+ phenotype ofagl mutants on alfalfa is due to these additional functions. Loci found by DNA sequencing to be adjacent toaglEFGAK include a probable regulatory gene (aglR ),zwf andedd , which encode the first two enzymes of the Entner-Doudoroff pathway,pgl , which shows homology to a gene encoding a putative phosphogluconolactonase, and a novelRhizobium -specific repeat element.

A Novel Sinorhizobium meliloti Operon Encodes an α-Glucosidase and a Periplasmic-Binding-Protein-Dependent Transport System for α-Glucosides