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whiE is a complex locus that specifies the polyketide spore pigment inStreptomyces coelicolor A3(2). Two divergently oriented promoters,whiEP1 andwhiEP2 , were identified in thewhiE gene cluster, and their activities were analyzed during colony development in wild-type and sporulation-deficient strains. Both promoters were developmentally regulated;whiEP1 andwhiEP2 transcripts were detected transiently at approximately the time when sporulation septa were observed in the aerial hyphae, and transcription from both promoters depended on each of the six known “early”whi genes required for sporulation septum formation (whiA , -B , -G , -H , -I , and -J ). Mutation of the late sporulation-specific sigma factor gene,sigF , had no effect on the activity ofwhiEP1 but blocked transcription fromwhiEP2 . However, ςF -containing holoenzyme was not sufficient to direct transcription ofwhiEP2 in vitro. ThewhiEP2 promoter controls expression ofwhiE ORFVIII, encoding a putative flavin adenine dinucleotide-dependent hydroxylase that catalyzes a late tailoring step in the spore pigment biosynthetic pathway. Disruption ofwhiE ORFVIII causes a change in spore color, from grey to greenish (T.-W. Yu and D. A. Hopwood, Microbiology 141:2779–2791, 1995). Consistent with these observations, construction of asigF null mutant ofS. coelicolor M145 caused the same change in spore color, showing that disruption ofsigF inS. coelicolor changes the nature of the spore pigment rather than preventing its synthesis altogether.

Developmental Regulation of Transcription of whiE , a Locus Specifying the Polyketide Spore Pigment in Streptomyces coelicolor A3(2)