English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

InAcinetobacter sp. strain ADP1, benzoate degradation requires theben genes for converting benzoate to catechol and thecat genes for degrading catechol. Here we describe a novel transcriptional activator, BenM, that regulates the chromosomalben andcat genes. BenM is homologous to CatM, a LysR-type transcriptional activator of thecat genes. Unusual regulatory features of this system include the abilities of both BenM and CatM to recognize the same inducer,cis ,cis -muconate, and to regulate some of the same genes, such ascatA andcatB . Unlike CatM, BenM responded to benzoate. Benzoate together withcis ,cis -muconate increased the BenM-dependent expression of thebenABCDE operon synergistically. CatM was not required for this synergism, nor did CatM regulate the expression of a chromosomalbenA ::lacZ transcriptional fusion. BenM-mediated regulation differs significantly from that of the TOL plasmid-encoded conversion of benzoate to catechol in pseudomonads. ThebenM gene is immediately upstream of, and divergently transcribed from,benA , and a possible DNA binding site for BenM was identified between the two coding regions. Two mutations in the predicted operator/promoter region renderedben gene expression either constitutive or inducible bycis ,cis -muconate but not benzoate. Mutants lacking BenM, CatM, or both of these regulators degraded aromatic compounds at different rates, and the levels of intermediary metabolites that accumulated depended on the genetic background. These studies indicated that BenM is necessary forben gene expression but not for expression of thecat genes, which can be regulated by CatM. In acatM -disrupted strain, BenM was able to induce higher levels ofcatA expression thancatB expression.

Regulation of Benzoate Degradation in Acinetobacter sp. Strain ADP1 by BenM, a LysR-Type Transcriptional Activator