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Candida albicans is the major fungal pathogen in humans, yet little is known about transcriptional regulation in this organism. Therefore, we have isolated, characterized, and expressed theC. albicans TATA-binding protein (TBP) gene (TBP1 ), because this general transcription initiation factor plays a key role in the activation and regulation of eukaryotic promoters. Southern and Northern blot analyses suggest that a singleC. albicans TBP1 locus is expressed at similar levels in the yeast and hyphal forms of this fungus. TheTBP1 open reading frame is 716 bp long and encodes a functional TBP of 27 kDa.C. albicans TBP is capable of binding specifically to a TATA box in vitro, substituting for the human TBP to activate basal transcription in vitro, and suppressing the lethal Δspt15 mutation inSaccharomyces cerevisiae . The predicted amino acid sequences of TBPs fromC. albicans and other organisms reveal a striking pattern of C-terminal conservation and N-terminal variability: the C-terminal DNA-binding domain displays at least 80% amino acid sequence identity to TBPs from fungi, flies, nematodes, slime molds, plants, and humans. Sequence differences between human and fungal TPBs in the DNA-binding domain may represent potential targets for antifungal therapy.

The TATA-Binding Protein (TBP) from the Human Fungal Pathogen Candida albicans Can Complement Defects in Human and Yeast TBPs