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Expression of cloned genes from isopropyl-β-d -thiogalactopyranoside (IPTG)-regulated promoters is lowered when theEscherichia coli CmlA/Cmr/MdfA efflux pump is overexpressed, probably due to IPTG exclusion from the cytoplasm. The previously reportedcmlA1 mutation confers a similar phenotype.cmlA1 contains an IS30 insertion upstream ofcmr/mdfA , which creates a putative promoter. CmlA overproduction also causes spectinomycin hypersensitivity.

journal of bacteriology

The <i>Escherichia coli cmlA</i> Gene Encodes the Multidrug Efflux Pump Cmr/MdfA and Is Responsible for Isopropyl-β- <scp>d</scp> -Thiogalactopyranoside Exclusion and Spectinomycin Sensitivity

The Escherichia coli cmlA Gene Encodes the Multidrug Efflux Pump Cmr/MdfA and Is Responsible for Isopropyl-β- d -Thiogalactopyranoside Exclusion and Spectinomycin Sensitivity