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Three open reading frames (ORFs) have been located downstream ofcefE in the cephamycin C gene cluster ofStreptomyces clavuligerus . ORF13 (pcd ) encodes a 496-amino-acid protein (molecular weight [MW], 52,488) with an N-terminal amino acid sequence identical to that of pure piperideine-6-carboxylate dehydrogenase. ORF14 (cmcT ) encodes a 523-amino-acid protein (MW, 54,232) analogous toStreptomyces proteins for efflux and resistance to antibiotics. ORF15 (pbp74 ) encodes a high molecular weight penicillin-binding protein (MW, 74,094).

journal of bacteriology

The <i>pcd</i> Gene Encoding Piperideine-6-Carboxylate Dehydrogenase Involved in Biosynthesis of α-Aminoadipic Acid Is Located in the Cephamycin Cluster of <i>Streptomyces clavuligerus</i>

The pcd Gene Encoding Piperideine-6-Carboxylate Dehydrogenase Involved in Biosynthesis of α-Aminoadipic Acid Is Located in the Cephamycin Cluster of Streptomyces clavuligerus