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The genomic DNA of the BE strain ofEscherichia coli has been scrutinized to detect porin genes that have not been identified so far. Southern blot analysis yielded two DNA segments which proved highly homologous to, yet distinct from, theompC ,ompF , andphoE porin genes. The two genes were cloned and sequenced. One of them, designatedompN , encodes a porin which, due to low levels of expression, has eluded prior identification. The functional properties (single-channel conductance) of the OmpN porin, purified to homogeneity, closely resemble those of the OmpC porin fromE. coli K-12. The second DNA fragment detected corresponds to thenmpC gene, which, due to an insertion of an IS1 element in its coding region, is not expressed inE. coli BE .

Identification and Characterization of Two Quiescent Porin Genes, nmpC and ompN , in Escherichia coli B E

Identification and Characterization of Two Quiescent Porin Genes, nmpC and ompN , in Escherichia coli B ^E