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Late in its growth cycle, transcription of the phage Mumom promoter (Pmom ) is activated by the phage gene product, C, a site-specific DNA binding protein. In vitro transcription analyses showed that this activation does not require specific contacts between C and the carboxyl-terminal region of the α or ς70 subunit ofEscherichia coli RNA polymerase. Unexpectedly, these results are in contrast to those known for another Mu-encoded transcriptional activator, Mor, which has a high degree of sequence identity with C and appears to interact with the carboxyl termini of both α and ς70 .

journal of bacteriology

Activation of Bacteriophage Mu mom Transcription by C Protein Does Not Require Specific Interaction with the Carboxyl-Terminal Region of the α or ς 70 Subunit of Escherichia coli RNA Polymerase