Open AccessCharacterization of iucA and iucC genes of the aerobactin system of plasmid ColV-K30 in Escherichia coli
Author(s) -
Vı́ctor de Lorenzo,
J. B. Neilands
Publication year - 1986
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.167.1.350-355.1986
Subject(s) - aerobactin , biology , plasmid , escherichia coli , complementation , gene , mutagenesis , mutant , genetics , dna , microbiology and biotechnology , enterobacteriaceae
A cloned 8.3-kilobase-pair DNA fragment carrying all the genes (iucABCD iutA) of the aerobactin iron transport system of plasmid pColV-K30 was subjected to in vitro mutagenesis to afford mutant genes iucA, iucC, and iucA iucC. Complementation analyses and identification of aerobactin precursors accumulated by Escherichia coli cells harboring the different constructions allowed assignment of the iucA and iucC genes to discrete steps in biosynthesis of the siderophore from N epsilon-acetyl-N epsilon-hydroxylysine and citrate. Plasmid pVLN10, a derivative carrying a DNA fragment complementing an iucC mutation, expressed in a minicell system a single 62,000-dalton protein as the product of this gene.